The E3 ubiquitin ligase UBR5 interacts with the H/ACA ribonucleoprotein complex and regulates ribosomal RNA biogenesis in embryonic stem cells.

UBR5 is an E3 ubiquitin ligase involved in distinct processes such as transcriptional regulation and development. UBR5 is highly upregulated in embryonic stem cells (ESCs), whereas its expression decreases with differentiation, suggesting a role for UBR5 in ESC function. However, little is known about how UBR5 regulates ESC identity. Here, ...
we define the protein interactome of UBR5 in ESCs and find interactions with distinct components of the H/ACA ribonucleoprotein complex, which is required for proper maturation of ribosomal RNA (rRNA). Notably, loss of UBR5 induces an abnormal accumulation of rRNA processing intermediates, resulting in diminished ribosomal levels. Consequently, lack of UBR5 triggers an increase in p53 levels and a concomitant decrease in cellular proliferation rates. Thus, our results indicate a link between UBR5 and rRNA maturation.
Mesh Terms:
Animals, Cells, Cultured, Embryonic Stem Cells, HEK293 Cells, Humans, Mice, RNA Processing, Post-Transcriptional, RNA, Ribosomal, Ribonucleoproteins, Tumor Suppressor Protein p53, Ubiquitin-Protein Ligases
FEBS Lett.
Date: Dec. 01, 2019
Download Curated Data For This Publication
223727
Switch View:
  • Interactions 106