P-glycoprotein is localized in caveolae in resistant cells and in brain capillaries.

A significant proportion of P-glycoprotein (P-gp) and caveolin was co-localized in caveolae isolated from resistant (CH(R)C5) cells overexpressing P-gp and from drug-sensitive Chinese hamster ovary cells (AuxB1). The proportion of P-gp and caveolin associated with caveolar microdomains was higher in CH(R)C5 cells grown in the presence of P-gp substrates (cyclosporin ...
A or colchicine) than in untreated CH(R)C5 cells. Coimmunoprecipitation of P-gp and caveolin from CH(R)C5 lysates suggests that there is a physical interaction between them. Furthermore, co-localization of P-gp and caveolin was found in caveolae from brain capillaries, indicating that this association also takes place in vivo.
Mesh Terms:
ATP Binding Cassette Transporter, Subfamily B, Member 1, Amino Acid Sequence, Animals, Brain, CHO Cells, Capillaries, Cricetinae, Humans, Membrane Proteins, Precipitin Tests, Tumor Cells, Cultured
FEBS Lett.
Date: Jan. 28, 2000
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