Network-Based Combinatorial CRISPR-Cas9 Screens Identify Synergistic Modules in Human Cells.
Tumorigenesis is a complex process that is driven by a combination of networks of genes and environmental factors; however, efficient approaches to identifying functional networks that are perturbed by the process of tumorigenesis are lacking. In this study, we provide a comprehensive network-based strategy for the systematic discovery of functional ... synergistic modules that are causal determinants of inflammation-induced tumorigenesis. Our approach prioritizes candidate genes selected by integrating clinical-based and network-based genome-wide gene prediction methods and identifies functional synergistic modules based on combinatorial CRISPR-Cas9 screening. On the basis of candidate genes inferred de novo from experimental and computational methods to be involved in inflammation and cancer, we used an existing TGF?1-induced cellular transformation model in colonic epithelial cells and a new combinatorial CRISPR-Cas9 screening strategy to construct an inflammation-induced differential genetic interaction network. The inflammation-induced differential genetic interaction network that we generated yielded functional insights into the genes and functional module combinations, and showed varied responses to the inflammation agents as well as active traditional Chinese medicine compounds. We identified opposing differential genetic interactions of inflammation-induced tumorigenesis: synergistic promotion and suppression. The synergistic promotion state was primarily caused by deletions in the immune and metabolism modules; the synergistic suppression state was primarily induced by deletions in the proliferation and immune modules or in the proliferation and metabolism modules. These results provide insight into possible early combinational targets and biomarkers for inflammation-induced tumorigenesis and highlight the synergistic effects that occur among immune, proliferation, and metabolism modules. In conclusion, this approach deepens the understanding of the underlying mechanisms that cause inflammation to potentially increase the cancer risk of colonic epithelial cells and accelerate the translation into novel functional modules or synergistic module combinations that modulate complex disease phenotypes.
Mesh Terms:
CRISPR-Cas Systems, Carcinogenesis, Cell Survival, Cell Transformation, Neoplastic, Colorectal Neoplasms, Computational Biology, Drug Discovery, Drug Screening Assays, Antitumor, Gene Editing, Gene Knockout Techniques, Gene Regulatory Networks, HEK293 Cells, Humans, Inflammation, Inflammatory Bowel Diseases, RNA Interference, Transcriptome, Transfection, Transforming Growth Factor beta1
CRISPR-Cas Systems, Carcinogenesis, Cell Survival, Cell Transformation, Neoplastic, Colorectal Neoplasms, Computational Biology, Drug Discovery, Drug Screening Assays, Antitumor, Gene Editing, Gene Knockout Techniques, Gene Regulatory Networks, HEK293 Cells, Humans, Inflammation, Inflammatory Bowel Diseases, RNA Interference, Transcriptome, Transfection, Transforming Growth Factor beta1
ACS Synth Biol
Date: Dec. 15, 2018
PubMed ID: 30762338
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