Structure of the yeast Swi/Snf complex in a nucleosome free state.
SWI/SNF remodelers play a key role in regulating chromatin architecture and gene expression. Here, we report the cryo-EM structure of the Saccharomyces cerevisiae Swi/Snf complex in a nucleosome-free state. The structure consists of a stable triangular base module and a flexible Arp module. The conserved subunits Swi1 and Swi3 form ... the backbone of the complex and closely interact with other components. Snf6, which is specific for yeast Swi/Snf complex, stabilizes the binding of the ATPase-containing subunit Snf2 to the base module. Comparison of the yeast Swi/Snf and RSC complexes reveals conserved structural features that govern the assembly and function of these two subfamilies of chromatin remodelers. Our findings complement those from recent structures of the yeast and human chromatin remodelers and provide further insights into the assembly and function of the SWI/SNF remodelers.
Mesh Terms:
Adenosine Triphosphatases, Chromatin, Chromosomal Proteins, Non-Histone, Cryoelectron Microscopy, DNA-Binding Proteins, Humans, Nuclear Proteins, Nucleosomes, Protein Binding, Protein Domains, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors
Adenosine Triphosphatases, Chromatin, Chromosomal Proteins, Non-Histone, Cryoelectron Microscopy, DNA-Binding Proteins, Humans, Nuclear Proteins, Nucleosomes, Protein Binding, Protein Domains, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors
Nat Commun
Date: Dec. 07, 2019
PubMed ID: 32636384
View in: Pubmed Google Scholar
Download Curated Data For This Publication
225901
Switch View:
- Interactions 11