Presenilin/?-secretase-dependent EphA3 processing mediates axon elongation through non-muscle myosin IIA.

EphA/ephrin signaling regulates axon growth and guidance of neurons, but whether this process occurs also independently of ephrins is unclear. We show that presenilin-1 (PS1)/?-secretase is required for axon growth in the developing mouse brain. PS1/?-secretase mediates axon growth by inhibiting RhoA signaling and cleaving EphA3 independently of ligand to ...
generate an intracellular domain (ICD) fragment that reverses axon defects in PS1/?-secretase- and EphA3-deficient hippocampal neurons. Proteomic analysis revealed that EphA3 ICD binds to non-muscle myosin IIA (NMIIA) and increases its phosphorylation (Ser1943), which promotes NMIIA filament disassembly and cytoskeleton rearrangement. PS1/?-secretase-deficient neurons show decreased phosphorylated NMIIA and NMIIA/actin colocalization. Moreover, pharmacological NMII inhibition reverses axon retraction in PS-deficient neurons suggesting that NMIIA mediates PS/EphA3-dependent axon elongation. In conclusion, PS/?-secretase-dependent EphA3 cleavage mediates axon growth by regulating filament assembly through RhoA signaling and NMIIA, suggesting opposite roles of EphA3 on inhibiting (ligand-dependent) and promoting (receptor processing) axon growth in developing neurons.
Mesh Terms:
Animals, Axons, Cells, Cultured, Humans, Mice, Nonmuscle Myosin Type IIA, Presenilin-1, Receptor, EphA3, Signal Transduction, rhoA GTP-Binding Protein
Elife
Date: Dec. 02, 2018
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