Functions for Cdc42p BEM adaptors in regulating a differentiation-type MAP kinase pathway.
Ras homology (Rho) GTPases regulate cell polarity and signal transduction pathways to control morphogenetic responses in different settings. In yeast, the Rho GTPase Cdc42p regulates cell polarity, and through the p21-activated kinase Ste20p, Cdc42p also regulates mitogen-activated protein kinase (MAPK) pathways (mating, filamentous growth or fMAPK, and HOG). Although much ... is known about how Cdc42p regulates cell polarity and the mating pathway, how Cdc42p regulates the fMAPK pathway is not clear. To address this question, Cdc42p-dependent MAPK pathways were compared in the filamentous (?1278b) strain background. Each MAPK pathway showed a unique activation profile, with the fMAPK pathway exhibiting slow activation kinetics compared with the mating and HOG pathways. A previously characterized version of Cdc42p, Cdc42pE100A, that is specifically defective for fMAPK pathway signaling, was defective for interaction with Bem4p, the pathway-specific adaptor for the fMAPK pathway. Corresponding residues in Bem4p were identified that were required for interaction with Cdc42p and fMAPK pathway signaling. The polarity adaptor Bem1p also regulated the fMAPK pathway. Versions of Bem1p defective for recruitment of Ste20p to the plasma membrane, intramolecular interactions, and interaction with the GEF, Cdc24p, were defective for fMAPK pathway signaling. Bem1p also regulated effector pathways in different ways. In some pathways, multiple domains of the protein were required for its function, whereas in other pathways, a single domain or function was needed. Genetic suppression tests showed that Bem4p and Bem1p regulate the fMAPK pathway in an ordered sequence. Collectively, the study demonstrates unique and sequential functions for Rho GTPase adaptors in regulating MAPK pathways.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Cell Polarity, GTP Phosphohydrolases, Guanine Nucleotide Exchange Factors, Intracellular Signaling Peptides and Proteins, MAP Kinase Kinase Kinases, MAP Kinase Signaling System, Mitogen-Activated Protein Kinases, Protein Binding, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, cdc42 GTP-Binding Protein, cdc42 GTP-Binding Protein, Saccharomyces cerevisiae, p21-Activated Kinases, rho GTP-Binding Proteins
Adaptor Proteins, Signal Transducing, Cell Polarity, GTP Phosphohydrolases, Guanine Nucleotide Exchange Factors, Intracellular Signaling Peptides and Proteins, MAP Kinase Kinase Kinases, MAP Kinase Signaling System, Mitogen-Activated Protein Kinases, Protein Binding, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, cdc42 GTP-Binding Protein, cdc42 GTP-Binding Protein, Saccharomyces cerevisiae, p21-Activated Kinases, rho GTP-Binding Proteins
Mol Biol Cell
Date: Dec. 15, 2019
PubMed ID: 31940256
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