Structure of eukaryotic DNA polymerase ? bound to the PCNA clamp while encircling DNA.

The DNA polymerase (Pol) ? of Saccharomyces cerevisiae (S.c.) is composed of the catalytic subunit Pol3 along with two regulatory subunits, Pol31 and Pol32. Pol ? binds to proliferating cell nuclear antigen (PCNA) and functions in genome replication, repair, and recombination. Unique among DNA polymerases, the Pol3 catalytic subunit contains ...
a 4Fe-4S cluster that may sense the cellular redox state. Here we report the 3.2-A cryo-EM structure of S.c. Pol ? in complex with primed DNA, an incoming ddTTP, and the PCNA clamp. Unexpectedly, Pol ? binds only one subunit of the PCNA trimer. This singular yet extensive interaction holds DNA such that the 2-nm-wide DNA threads through the center of the 3-nm interior channel of the clamp without directly contacting the protein. Thus, a water-mediated clamp and DNA interface enables the PCNA clamp to "waterskate" along the duplex with minimum drag. Pol31 and Pol32 are positioned off to the side of the catalytic Pol3-PCNA-DNA axis. We show here that Pol31-Pol32 binds single-stranded DNA that we propose underlies polymerase recycling during lagging strand synthesis, in analogy to Escherichia coli replicase. Interestingly, the 4Fe-4S cluster in the C-terminal CysB domain of Pol3 forms the central interface to Pol31-Pol32, and this strategic location may explain the regulation of the oxidation state on Pol ? activity, possibly useful during cellular oxidative stress. Importantly, human cancer and other disease mutations map to nearly every domain of Pol3, suggesting that all aspects of Pol ? replication are important to human health and disease.
Mesh Terms:
Cryoelectron Microscopy, DNA, DNA Polymerase III, Dideoxynucleotides, Iron-Sulfur Proteins, Models, Molecular, Mutation, Neoplasms, Proliferating Cell Nuclear Antigen, Protein Binding, Protein Subunits, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Thymine Nucleotides
Proc Natl Acad Sci U S A
Date: Dec. 01, 2019
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