Cloning and characterization of cell adhesion kinase beta, a novel protein-tyrosine kinase of the focal adhesion kinase subfamily.

A second protein-tyrosine kinase (PTK) of the focal adhesion kinase (FAK) subfamily, cell adhesion kinase beta (CAK beta), was identified by cDNA cloning. The rat CAK beta is a 115.7-kDa PTK that contains N- and C-terminal domains of 418 and 330 amino acid residues besides the central kinase domain. The ...
rat CAK beta has a homology with mouse FAK over their entire lengths except for the extreme N-terminal 88 residues and shares 45% overall sequence identity (60% identical in the catalytic domain), which indicates that CAK beta is a protein structurally related to but different from FAK. The CAK beta gene is less evenly expressed in a variety of rat organs than the FAK gene. Anti-CAK beta antibody immunoprecipitated a 113-kDa protein from rat brain, 3Y1 fibroblasts, and COS-7 cells transfected with CAK beta cDNA. The tyrosine-phosphorylated state of CAK beta was not reduced on trypsinization, nor enhanced in response to plating 3Y1 cells onto fibronectin. CAK beta localized to sites of cell-to-cell contact in COS-7 transfected with CAK beta cDNA, in which FAK was found at the bottom of the cells. Thus, CAK beta is a PTK possibly participating in the signal transduction regulated by cell-to-cell contacts.
Mesh Terms:
Amino Acid Sequence, Animals, Base Sequence, Brain, Cell Adhesion Molecules, Cell Line, Cloning, Molecular, DNA, Complementary, Focal Adhesion Kinase 1, Focal Adhesion Kinase 2, Focal Adhesion Protein-Tyrosine Kinases, Humans, Molecular Sequence Data, Phosphorylation, Protein-Tyrosine Kinases, RNA, Messenger, Sequence Homology, Amino Acid, Tumor Cells, Cultured, Tyrosine
J Biol Chem
Date: Sep. 08, 1995
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