Deletion of yeast TPK1 reduces the efficiency of non-homologous end joining DNA repair.
Non-homologous end joining (NHEJ) is a highly conserved mechanism of DNA double-stranded break (DSB) repair. Here we utilize a computational protein-protein interaction method to identify human PRKACB as a potential candidate interacting with NHEJ proteins. We show that the deletion of its yeast homolog, TPK1 that codes for the protein ... kinase A catalytic subunit reduces the efficiency of NHEJ repair of breaks with overhangs and blunt ends in plasmid-based repair assays. Additionally, tpk1? mutants showed defects in the repair of chromosomal breaks induced by HO-site specific endonuclease. Our double deletion mutant analyses suggest that TPK1 and YKU80, a key player in NHEJ could function in parallel pathways. Altogether, here we report a novel involvement for TPK1 in NHEJ.
Mesh Terms:
Cyclic AMP-Dependent Protein Kinase Catalytic Subunits, Cyclic AMP-Dependent Protein Kinases, DNA Breaks, Double-Stranded, DNA End-Joining Repair, DNA, Fungal, DNA-Binding Proteins, Gene Deletion, Genes, Fungal, Genes, Synthetic, Genetic Association Studies, Humans, Protein Interaction Maps, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Cyclic AMP-Dependent Protein Kinase Catalytic Subunits, Cyclic AMP-Dependent Protein Kinases, DNA Breaks, Double-Stranded, DNA End-Joining Repair, DNA, Fungal, DNA-Binding Proteins, Gene Deletion, Genes, Fungal, Genes, Synthetic, Genetic Association Studies, Humans, Protein Interaction Maps, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Biochem Biophys Res Commun
Date: Dec. 17, 2019
PubMed ID: 33008596
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