SENP1 mediates TNF-induced desumoylation and cytoplasmic translocation of HIPK1 to enhance ASK1-dependent apoptosis.
We have previously shown that tumor necrosis factor (TNF)-induced desumoylation and subsequent cytoplasmic translocation of HIPK1 are critical for ASK1-JNK activation. However, the mechanism by which TNF induces desumoylation of HIPK1 is unclear. Here, we show that SENP1, a SUMO-specific protease, specifically deconjugates SUMO from HIPK1 in vitro and in ... vivo. In resting endothelial cells (ECs), SENP1 is localized in the cytoplasm where it is complexed with an antioxidant protein thioredoxin. TNF induces the release of SENP1 from thioredoxin as well as nuclear translocation of SENP1. TNF-induced SENP1 nuclear translocation is specifically blocked by antioxidants such as N-acetyl-cysteine, suggesting that TNF-induced translocation of SENP1 is ROS dependent. TNF-induced nuclear import of SENP1 kinetically correlates with HIPK1 desumoylation and cytoplasmic translocation. Furthermore, the wild-type form of SENP1 enhances, whereas the catalytic-inactive mutant form or siRNA of SENP1 blocks, TNF-induced desumoylation and cytoplasmic translocation of HIPK1 as well as TNF-induced ASK1-JNK activation. More importantly, these critical functions of SENP1 in TNF signaling were further confirmed in mouse embryonic fibroblast cells derived from SENP1-knockout mice. We conclude that SENP1 mediates TNF-induced desumoylation and translocation of HIPK1, leading to an enhanced ASK1-dependent apoptosis.
Mesh Terms:
Acetylcysteine, Animals, Antioxidants, Apoptosis, Carrier Proteins, Cattle, Cells, Cultured, Cysteine Endopeptidases, Cytoplasm, Endopeptidases, Endothelial Cells, Fibroblasts, Humans, MAP Kinase Kinase Kinase 5, Mice, Mice, Knockout, Mutation, Protein Kinases, Protein Processing, Post-Translational, Protein Transport, Protein-Serine-Threonine Kinases, RNA Interference, RNA, Small Interfering, Reactive Oxygen Species, Recombinant Proteins, Signal Transduction, Small Ubiquitin-Related Modifier Proteins, Thioredoxins, Time Factors, Transfection, Tumor Necrosis Factor-alpha
Acetylcysteine, Animals, Antioxidants, Apoptosis, Carrier Proteins, Cattle, Cells, Cultured, Cysteine Endopeptidases, Cytoplasm, Endopeptidases, Endothelial Cells, Fibroblasts, Humans, MAP Kinase Kinase Kinase 5, Mice, Mice, Knockout, Mutation, Protein Kinases, Protein Processing, Post-Translational, Protein Transport, Protein-Serine-Threonine Kinases, RNA Interference, RNA, Small Interfering, Reactive Oxygen Species, Recombinant Proteins, Signal Transduction, Small Ubiquitin-Related Modifier Proteins, Thioredoxins, Time Factors, Transfection, Tumor Necrosis Factor-alpha
Cell Death Differ
Date: Apr. 01, 2008
PubMed ID: 18219322
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