Nuclear envelope-vacuole contacts mitigate nuclear pore complex assembly stress.
The intricacy of nuclear pore complex (NPC) biogenesis imposes risks of failure that can cause defects in nuclear transport and nuclear envelope (NE) morphology; however, cellular mechanisms used to alleviate NPC assembly stress are not well defined. In the budding yeast Saccharomyces cerevisiae, we demonstrate that NVJ1- and MDM1-enriched NE-vacuole ... contacts increase when NPC assembly is compromised in several nup mutants, including nup116?GLFG cells. These interorganelle nucleus-vacuole junctions (NVJs) cooperate with lipid droplets to maintain viability and enhance NPC formation in assembly mutants. Additionally, NVJs function with ATG1 to remodel the NE and promote vacuole-dependent degradation of specific nucleoporins in nup116?GLFG cells. Importantly, NVJs significantly improve the physiology of NPC assembly mutants, despite having only negligible effects when NPC biogenesis is unperturbed. These results therefore define how NE-vacuole interorganelle contacts coordinate responses to mitigate deleterious cellular effects caused by disrupted NPC assembly.
Mesh Terms:
Gene Deletion, Intermediate Filament Proteins, Nuclear Pore, Nuclear Pore Complex Proteins, Receptors, Cytoplasmic and Nuclear, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Vacuoles
Gene Deletion, Intermediate Filament Proteins, Nuclear Pore, Nuclear Pore Complex Proteins, Receptors, Cytoplasmic and Nuclear, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Vacuoles
J Cell Biol
Date: Dec. 07, 2019
PubMed ID: 33053148
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