Inhibitor of Growth 4 (ING4) is a positive regulator of rRNA synthesis.
Ribosome biogenesis is essential for maintaining basic cellular activities although its mechanism is not fully understood. Inhibitor of growth 4 (ING4) is a member of ING family while its cellular functions remain controversial. Here, we identified several nucleolar proteins as novel ING4 interacting proteins. ING4 localized in the nucleus with ... strong accumulation in the nucleolus through its plant homeodomain, which is known to interact with histone trimethylated H3K4, commonly present in the promoter of active genes. ING4 deficient cells exhibited slower proliferation and the alteration in nucleolar structure with reduced rRNA transcription, which was rescued by exogenous expression of GFP-ING4 to the similar levels of wild type cells. In the ING4 deficient cells, histone H3K9 acetylation and the key rRNA transcription factor UBF at the promoter of rDNA were reduced, both of which were also recovered by exogenous GFP-ING4 expression. Thus, ING4 could positively regulate rRNA transcription through modulation of histone modifications at the rDNA promoter.
Mesh Terms:
Acetylation, Cell Cycle Proteins, Cell Line, Tumor, Cell Nucleus, DNA, Ribosomal, HeLa Cells, Histones, Homeodomain Proteins, Humans, Promoter Regions, Genetic, RNA, Ribosomal, Transcription, Genetic, Tumor Suppressor Proteins
Acetylation, Cell Cycle Proteins, Cell Line, Tumor, Cell Nucleus, DNA, Ribosomal, HeLa Cells, Histones, Homeodomain Proteins, Humans, Promoter Regions, Genetic, RNA, Ribosomal, Transcription, Genetic, Tumor Suppressor Proteins
Sci Rep
Date: Dec. 21, 2018
PubMed ID: 31754246
View in: Pubmed Google Scholar
Download Curated Data For This Publication
228203
Switch View:
- Interactions 15