Blocking PPAR? interaction facilitates Nur77 interdiction of fatty acid uptake and suppresses breast cancer progression.

Nuclear receptor Nur77 participates in multiple metabolic regulations and plays paradoxical roles in tumorigeneses. Herein, we demonstrated that the knockout of Nur77 stimulated mammary tumor development in two mouse models, which would be reversed by a specific reexpression of Nur77 in mammary tissues. Mechanistically, Nur77 interacted and recruited corepressors, the ...
SWI/SNF complex, to the promoters of CD36 and FABP4 to suppress their transcriptions, which hampered the fatty acid uptake, leading to the inhibition of cell proliferation. Peroxisome proliferator-activated receptor-? (PPAR?) played an antagonistic role in this process through binding to Nur77 to facilitate ubiquitin ligase Trim13-mediated ubiquitination and degradation of Nur77. Cocrystallographic and functional analysis revealed that Csn-B, a Nur77-targeting compound, promoted the formation of Nur77 homodimer to prevent PPAR? binding by steric hindrance, thereby strengthening the Nur77's inhibitory role in breast cancer. Therefore, our study reveals a regulatory function of Nur77 in breast cancer via impeding fatty acid uptake.
Mesh Terms:
Adult, Aged, Aged, 80 and over, Animals, Breast, Breast Neoplasms, Cell Proliferation, DNA-Binding Proteins, Disease Models, Animal, Disease Progression, Fatty Acids, Female, Humans, Kaplan-Meier Estimate, Lipid Metabolism, Mammary Glands, Animal, Mice, Middle Aged, Nuclear Receptor Subfamily 4, Group A, Member 1, PPAR gamma, Phenylacetates, Primary Cell Culture, Prognosis, Proteolysis, Tissue Array Analysis, Tumor Cells, Cultured, Tumor Suppressor Proteins, Ubiquitination
Proc Natl Acad Sci U S A
Date: Dec. 03, 2019
Download Curated Data For This Publication
229315
Switch View:
  • Interactions 102