Production of ?-carotene in Saccharomyces cerevisiae through altering yeast lipid metabolism.

Saccharomyces cerevisiae is a widely used cell factory for the production of fuels and chemicals. However, as a non-oleaginous yeast, S. cerevisiae has a limited production capacity for lipophilic compounds, such as ?-carotene. To increase its accumulation of ?-carotene, we engineered different lipid metabolic pathways in a ?-carotene producing strain ...
and investigated the relationship between lipid components and the accumulation of ?-carotene. We found that overexpression of sterol ester synthesis genes ARE1 and ARE2 increased ?-carotene yield by 1.5-fold. Deletion of phosphatidate phosphatase (PAP) genes (PAH1, DPP1, and LPP1) also increased ?-carotene yield by twofold. Combining these two strategies resulted in a 2.4-fold improvement in ?-carotene production compared with the starting strain. These results demonstrated that regulating lipid metabolism pathways is important for ?-carotene accumulation in S. cerevisiae, and may also shed insights to the accumulation of other lipophilic compounds in yeast.
Biotechnol Bioeng
Date: Dec. 01, 2020
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