Properties of the co-chaperone protein p23 erroneously attributed to ALG-2 (apoptosis-linked gene 2).
A commercial antibody (clone 22) directed against the apoptosis-linked gene 2 (alg2, pdcd6) encoded protein has been used by several groups. Up-regulated expression of the antigen was observed in primary tumours and in metastatic tissue and also during rat brain ischemia. Furthermore, antigen down-regulation was found in human atherosclerotic plaques. ... Recently, we found that the clone 22 antibody does not recognise ALG-2. In the present study the antigen of the clone 22 antibody was identified as the heat shock protein 90 (HSP90) co-chaperone protein p23, identical to the cytosolic prostaglandin E2 synthase, by immunoprecipitation followed by tryptic in-gel digests and mass spectrometry of the purified peptides. Moreover, the heterogeneous ribonuclear protein A2/B1 was found to be a part of the p23 co-immunoprecipitated protein complex.
Mesh Terms:
Animals, Antibodies, Apoptosis, Apoptosis Regulatory Proteins, Calcium-Binding Proteins, Cloning, Molecular, Gene Expression Regulation, Humans, Intramolecular Oxidoreductases, Jurkat Cells, Mice, Molecular Chaperones, Peptide Fragments, Phosphoproteins, Precipitin Tests, Prostaglandin-E Synthases, Rats, Recombinant Proteins, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, fas Receptor
Animals, Antibodies, Apoptosis, Apoptosis Regulatory Proteins, Calcium-Binding Proteins, Cloning, Molecular, Gene Expression Regulation, Humans, Intramolecular Oxidoreductases, Jurkat Cells, Mice, Molecular Chaperones, Peptide Fragments, Phosphoproteins, Precipitin Tests, Prostaglandin-E Synthases, Rats, Recombinant Proteins, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, fas Receptor
FEBS Lett
Date: Dec. 18, 2003
PubMed ID: 14675759
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