The inositol polyphosphate 5-phosphatase Ocrl associates with endosomes that are partially coated with clathrin.

The subcellular localization of Ocrl, the inositol polyphosphate 5-phosphatase that is mutated in Lowe syndrome, was investigated by fluorescence microscopy. Ocrl was localized to endosomes and Golgi membranes along with clathrin, giantin, the mannose 6-phosphate receptor, transferrin, and the early endosomal antigen 1 endosomal marker in fixed cells. The endosomal ...
localization of Ocrl was confirmed by live-cell time-lapse microscopy in which we monitored the dynamics of Ocrl on endosomes. GST binding assays show that Ocrl interacts with the clathrin terminal domain and the clathrin adaptor protein AP-2. Our findings suggest a role for Ocrl in endosomal receptor trafficking and sorting.
Mesh Terms:
Adaptor Protein Complex 2, Animals, Brain, Cell Line, Clathrin, Endosomes, Fluorescent Dyes, Golgi Apparatus, Humans, Intracellular Membranes, Microscopy, Fluorescence, Phosphoric Monoester Hydrolases, Receptor, IGF Type 2
Proc Natl Acad Sci U S A
Date: Sep. 14, 2004
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