ASB16-AS1 up-regulated and phosphorylated TRIM37 to activate NF-?B pathway and promote proliferation, stemness, and cisplatin resistance of gastric cancer.
Long non-coding RNA (lncRNA) ASB16 antisense RNA 1 (ASB16-AS1) is recognized as an oncogene in several cancer types, but its relation to GC is unknown. Tripartite motif containing 37 (TRIM37) has been proven to accelerate the development of gastric cancer (GC), whereas the molecular mechanism assisted ASB16-AS1 and TRIM37 in ... regulating GC progression remains unclear.Differentially expressed lncRNAs in GC samples were analyzed based on Gene Expression Omnibus (GEO) data. CCK-8 and colony formation assays were applied to determine the proliferative ability of GC cells. Stem cell-like phenotype of GC cells was assessed by sphere formation assay and flow cytometry analysis. Luciferase reporter assay, RNA immunoprecipitation (RIP), pulldown, and co-immunoprecipitation (Co-IP) were performed to verify the interplay of RNA molecules.ASB16-AS1 was upregulated in GC samples according to GEO data and qRT-PCR analysis. ASB16-AS1 strengthened the proliferative ability and stem cell-like characteristics in GC cells. More importantly, ASB16-AS1 encouraged GC cell growth in vivo. Mechanistically, ASB16-AS1 strengthened TRIM37 expression by sequestering miR-3918 and miR-4676-3p. ASB16-AS1 activated NF-kappa B (NF-?B) pathway by cooperating with ATM serine/threonine kinase (ATM) to induce TRIM37 phosphorylation.In summary, ASB16-AS1 exerted oncogenic functions in GC through modulating TRIM37 expression at both mRNA and protein levels.
Mesh Terms:
Ankyrin Repeat, Carcinogenesis, Cell Culture Techniques, Cell Line, Tumor, Cell Proliferation, Cisplatin, Drug Resistance, Gene Expression Regulation, Neoplastic, Humans, MicroRNAs, NF-kappa B, Neoplastic Stem Cells, Phosphorylation, RNA, Long Noncoding, Real-Time Polymerase Chain Reaction, Signal Transduction, Stomach Neoplasms, Suppressor of Cytokine Signaling Proteins, Tripartite Motif Proteins, Ubiquitin-Protein Ligases, Up-Regulation
Ankyrin Repeat, Carcinogenesis, Cell Culture Techniques, Cell Line, Tumor, Cell Proliferation, Cisplatin, Drug Resistance, Gene Expression Regulation, Neoplastic, Humans, MicroRNAs, NF-kappa B, Neoplastic Stem Cells, Phosphorylation, RNA, Long Noncoding, Real-Time Polymerase Chain Reaction, Signal Transduction, Stomach Neoplasms, Suppressor of Cytokine Signaling Proteins, Tripartite Motif Proteins, Ubiquitin-Protein Ligases, Up-Regulation
Gastric Cancer
Date: Jan. 01, 2021
PubMed ID: 32572790
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