JIP4 is a PLK1 binding protein that regulates p38MAPK activity in G2 phase.
Cell cycle progression from G2 phase into mitosis is regulated by a complex network of mechanisms, all of which finally control the timing of Cyclin B/CDK1 activation. PLK1 regulates a network of events that contribute to regulating G2/M phase progression. Here we have used a proteomics approach to identify proteins ... that specifically bind to the Polobox domain of PLK1. This identified a panel of proteins that were either associated with PLK1 in G2 phase and/or mitosis, the strongest interaction being with the MAPK scaffold protein JIP4. PLK1 binding to JIP4 was found in G2 phase and mitosis, and PLK1 binding was self-primed by PLK1 phosphorylation of JIP4. PLK1 binding is required for JIP4-dependent p38MAPK activation in G2 phase during normal cell cycle progression, but not in either G2 phase or mitotic stress response. Finally, JIP4 is a target for caspase-dependent cleavage in mitotically arrested cells. The role for the PLK1-JIP4 regulated p38MAPK activation in G2 phase is unclear, but it does not affect either progression into or through mitosis.
Mesh Terms:
Adaptor Proteins, Signal Transducing, CDC2 Protein Kinase, Cell Cycle Proteins, Cell Line, Tumor, Cyclin B, Cyclin-Dependent Kinases, G2 Phase, HEK293 Cells, HeLa Cells, Humans, M Phase Cell Cycle Checkpoints, Mitosis, Phosphorylation, Protein Binding, Protein Serine-Threonine Kinases, Protein Structure, Tertiary, Proto-Oncogene Proteins, RNA Interference, RNA, Small Interfering, p38 Mitogen-Activated Protein Kinases
Adaptor Proteins, Signal Transducing, CDC2 Protein Kinase, Cell Cycle Proteins, Cell Line, Tumor, Cyclin B, Cyclin-Dependent Kinases, G2 Phase, HEK293 Cells, HeLa Cells, Humans, M Phase Cell Cycle Checkpoints, Mitosis, Phosphorylation, Protein Binding, Protein Serine-Threonine Kinases, Protein Structure, Tertiary, Proto-Oncogene Proteins, RNA Interference, RNA, Small Interfering, p38 Mitogen-Activated Protein Kinases
Cell Signal
Date: Nov. 01, 2015
PubMed ID: 26291670
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