EndoBind detects endogenous protein-protein interactions in real time.
We present two high-throughput compatible methods to detect the interaction of ectopically expressed (RT-Bind) or endogenously tagged (EndoBind) proteins of interest. Both approaches provide temporal evaluation of dimer formation over an extended duration. Using examples of the Nrf2-KEAP1 and the CRAF-KRAS-G12V interaction, we demonstrate that our method allows for the ... detection of signal for more than 2 days after substrate addition, allowing for continuous monitoring of endogenous protein-protein interactions in real time.
Mesh Terms:
HEK293 Cells, High-Throughput Screening Assays, Humans, Kelch-Like ECH-Associated Protein 1, NF-E2-Related Factor 2, Protein Binding, Proto-Oncogene Proteins p21(ras)
HEK293 Cells, High-Throughput Screening Assays, Humans, Kelch-Like ECH-Associated Protein 1, NF-E2-Related Factor 2, Protein Binding, Proto-Oncogene Proteins p21(ras)
Commun Biol
Date: Dec. 15, 2020
PubMed ID: 34526658
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