ZSWIM8 is a myogenic protein that partly prevents C2C12 differentiation.
Cell adhesion molecule-related/downregulated by oncogenes (Cdon) is a cell-surface receptor that mediates cell-cell interactions and positively regulates myogenesis. The cytoplasmic region of Cdon interacts with other proteins to form a Cdon/JLP/Bnip-2/CDC42 complex that activates p38 mitogen-activated protein kinase (MAPK) and induces myogenesis. However, Cdon complex may include other proteins during ... myogenesis. In this study, we found that Cullin 2-interacting protein zinc finger SWIM type containing 8 (ZSWIM8) ubiquitin ligase is induced during C2C12 differentiation and is included in the Cdon complex. We knocked-down Zswim8 in C2C12 cells to determine the effect of ZSWIM8 on differentiation. However, we detected neither ZSWIM8-dependent ubiquitination nor the degradation of Bnip2, Cdon, or JLP. In contrast, ZSWIM8 knockdown accelerated C2C12 differentiation. These results suggest that ZSWIM8 is a Cdon complex-included myogenic protein that prevents C2C12 differentiation without affecting the stability of Bnip2, Cdon, and JLP.
Mesh Terms:
Animals, Cell Adhesion Molecules, Cell Differentiation, Cell Line, Cell Line, Tumor, HEK293 Cells, Humans, K562 Cells, MAP Kinase Signaling System, Muscle Development, Protein Binding, Ubiquitin-Protein Ligases, p38 Mitogen-Activated Protein Kinases
Animals, Cell Adhesion Molecules, Cell Differentiation, Cell Line, Cell Line, Tumor, HEK293 Cells, Humans, K562 Cells, MAP Kinase Signaling System, Muscle Development, Protein Binding, Ubiquitin-Protein Ligases, p38 Mitogen-Activated Protein Kinases
Sci Rep
Date: Dec. 22, 2020
PubMed ID: 34686700
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