RNA-DNA hybrids regulate meiotic recombination.
RNA-DNA hybrids are often associated with genome instability and also function as a cellular regulator in many biological processes. In this study, we show that accumulated RNA-DNA hybrids cause multiple defects in budding yeast meiosis, including decreased sporulation efficiency and spore viability. Further analysis shows that these RNA-DNA hybrid foci ... colocalize with RPA/Rad51 foci on chromosomes. The efficient formation of RNA-DNA hybrid foci depends on Rad52 and ssDNA ends of meiotic DNA double-strand breaks (DSBs), and their number is correlated with DSB frequency. Interestingly, RNA-DNA hybrid foci and recombination foci show similar dynamics. The excessive accumulation of RNA-DNA hybrids around DSBs competes with Rad51/Dmc1, impairs homolog bias, and decreases crossover and noncrossover recombination. Furthermore, precocious removal of RNA-DNA hybrids by RNase H1 overexpression also impairs meiotic recombination similarly. Taken together, our results demonstrate that RNA-DNA hybrids form at ssDNA ends of DSBs to actively regulate meiotic recombination.
Mesh Terms:
Cell Cycle Proteins, DNA Breaks, Double-Stranded, DNA Breaks, Single-Stranded, DNA, Fungal, DNA-Binding Proteins, Homologous Recombination, Meiosis, Nucleic Acid Conformation, Nucleic Acid Heteroduplexes, RNA, Fungal, Rad51 Recombinase, Replication Protein A, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Cell Cycle Proteins, DNA Breaks, Double-Stranded, DNA Breaks, Single-Stranded, DNA, Fungal, DNA-Binding Proteins, Homologous Recombination, Meiosis, Nucleic Acid Conformation, Nucleic Acid Heteroduplexes, RNA, Fungal, Rad51 Recombinase, Replication Protein A, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Cell Rep
Date: Dec. 07, 2020
PubMed ID: 34879269
View in: Pubmed Google Scholar
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