Cyclin B1/Cdk1 binds and phosphorylates Filamin A and regulates its ability to cross-link actin.
Substantial actin remodelling occurs prior to mitosis as cells alter their shape in preparation for cytokinesis. In mammalian cells, mitosis is initiated by a heterodimer of cyclin B1 and the cyclin dependent kinase 1 (Cdk1) serine/threonine kinase. In this report. we show that human cyclin B1 binds the actin cross-linking ... protein Filamin-A (FLNa). The proteins co-immunoprecipitate and co-localize in mitotic human cells. We find that cyclin B1/Cdk1 can phosphorylate FLNa in vitro and reduce its ability to gelate actin. We have also identified serine 1436 as one FLNa residue phosphorylated by cyclin B1/Cdk1 in vitro. Our results suggest a role for cyclin B1/Cdk1 in FLNa-dependent actin remodelling.
Mesh Terms:
Actins, Amino Acid Sequence, CDC2 Protein Kinase, Contractile Proteins, Cyclin B, Cyclin B1, Filamins, Humans, Microfilament Proteins, Molecular Sequence Data, Phosphorylation, Protein Interaction Mapping, Serine, Two-Hybrid System Techniques
Actins, Amino Acid Sequence, CDC2 Protein Kinase, Contractile Proteins, Cyclin B, Cyclin B1, Filamins, Humans, Microfilament Proteins, Molecular Sequence Data, Phosphorylation, Protein Interaction Mapping, Serine, Two-Hybrid System Techniques
FEBS Lett
Date: Apr. 17, 2007
PubMed ID: 17408621
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