Yeast SKO1 gene encodes a bZIP protein that binds to the CRE motif and acts as a repressor of transcription.

We have cloned a yeast gene, SKO1, which in high copy number suppresses lethal overexpression of cAMP-dependent protein kinase. SKO1 encodes a bZIP protein that binds to the CRE motif, TGACGTCA. We found that SKO1 also binds to a CRE-like site in SUC2, a yeast gene encoding invertase which is ...
under positive control by cAMP. A disruption of the SKO1 gene causes a partial derepression of SUC2, indicating that SKO1 is a negative regulator of the SUC2 gene. SKO1 interacts positively with MIG1, a zinc finger protein that mediates glucose repression of SUC2. A kinetic analysis revealed a complex regulation of the SUC2 mRNA in response to glucose. First, MIG1 mediates a rapid and strong repression of SUC2, which is complete within 10 minutes. Second, a MIG1-independent process causes a further slow reduction in the mRNA. Third, in the absence of MIG1, there is also a rapid but transient glucose induction of the SUC2 mRNA. This induction is correlated with a transient loss of SKO1-dependent repression.
Mesh Terms:
Amino Acid Sequence, Base Sequence, Basic-Leucine Zipper Transcription Factors, Binding Sites, Blotting, Northern, Cloning, Molecular, DNA-Binding Proteins, G-Box Binding Factors, Gene Expression Regulation, Fungal, Genes, Fungal, Glucose, Glucosyltransferases, Leucine Zippers, Molecular Sequence Data, Oligonucleotide Probes, Plant Proteins, Plasmids, Promoter Regions, Genetic, Repressor Proteins, Restriction Mapping, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors, Transcription, Genetic
Nucleic Acids Res.
Date: Oct. 25, 1992
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