Ursodeoxycholic acid reduces antitumor immunosuppression by inducing CHIP-mediated TGF-? degradation.
TGF-? is essential for inducing systemic tumor immunosuppression; thus, blocking TGF-? can greatly enhance antitumor immunity. However, there are still no effective TGF-? inhibitors in clinical use. Here, we show that the clinically approved compound ursodeoxycholic acid (UDCA), by degrading TGF-?, enhances antitumor immunity through restraining Treg cell differentiation and ... activation in tumor-bearing mice. Furthermore, UDCA synergizes with anti-PD-1 to enhance antitumor immunity and tumor-specific immune memory in tumor-bearing mice. UDCA phosphorylates TGF-? at T282 site via TGR5-cAMP-PKA axis, causing increased binding of TGF-? to carboxyl terminus of Hsc70-interacting protein (CHIP). Then, CHIP ubiquitinates TGF-? at the K315 site, initiating p62-dependent autophagic sorting and subsequent degradation of TGF-?. Notably, results of retrospective analysis shows that combination therapy with anti-PD-1 or anti-PD-L1 and UDCA has better efficacy in tumor patients than anti-PD-1 or anti-PD-L1 alone. Thus, our results show a mechanism for TGF-? regulation and implicate UDCA as a potential TGF-? inhibitor to enhance antitumor immunity.
Mesh Terms:
Animals, Cell Line, Tumor, Humans, Immunosuppression Therapy, Mice, Neoplasms, Retrospective Studies, Transforming Growth Factor beta, Ursodeoxycholic Acid
Animals, Cell Line, Tumor, Humans, Immunosuppression Therapy, Mice, Neoplasms, Retrospective Studies, Transforming Growth Factor beta, Ursodeoxycholic Acid
Nat Commun
Date: Dec. 14, 2021
PubMed ID: 35701426
View in: Pubmed Google Scholar
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