A guard protein mediated quality control mechanism monitors 5'-capping of pre-mRNAs.

Efficient gene expression requires properly matured mRNAs for functional transcript translation. Several factors including the guard proteins monitor maturation and act as nuclear retention factors for unprocessed pre-mRNAs. Here we show that the guard protein Npl3 monitors 5'-capping. In its absence, uncapped transcripts resist degradation, because the Rat1-Rai1 5'-end degradation ...
factors are not efficiently recruited to these faulty transcripts. Importantly, in npl3?, these improperly capped transcripts escape this quality control checkpoint and leak into the cytoplasm. Our data suggest a model in which Npl3 associates with the Rai1 bound pre-mRNAs. In case the transcript was properly capped and is thus CBC (cap binding complex) bound, Rai1 dissociates from Npl3 allowing the export factor Mex67 to interact with this guard protein and support nuclear export. In case Npl3 does not detect proper capping through CBC attachment, Rai1 binding persists and Rat1 can join this 5'-complex to degrade the faulty transcript.
Mesh Terms:
Nuclear Proteins, RNA Caps, RNA Precursors, RNA-Binding Proteins, Saccharomyces cerevisiae Proteins
Nucleic Acids Res
Date: Oct. 28, 2022
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