SUMO-2 promotes mRNA translation by enhancing interaction between eIF4E and eIF4G.

Small ubiquitin-like modifier (SUMO) proteins regulate many important eukaryotic cellular processes through reversible covalent conjugation to target proteins. In addition to its many well-known biological consequences, like subcellular translocation of protein, subnuclear structure formation, and modulation of transcriptional activity, we show here that SUMO-2 also plays a role in mRNA ...
translation. SUMO-2 promoted formation of the active eukaryotic initiation factor 4F (eIF4F) complex by enhancing interaction between Eukaryotic Initiation Factor 4E (eIF4E) and Eukaryotic Initiation Factor 4G (eIF4G), and induced translation of a subset of proteins, such as cyclinD1 and c-myc, which essential for cell proliferation and apoptosis. As expected, overexpression of SUMO-2 can partially cancel out the disrupting effect of 4EGI-1, a small molecule inhibitor of eIF4E/eIF4G interaction, on formation of the eIF4F complex, translation of the cap-dependent protein, cell proliferation and apoptosis. On the other hand, SUMO-2 knockdown via shRNA partially impaired cap-dependent translation and cell proliferation and promoted apoptosis. These results collectively suggest that SUMO-2 conjugation plays a crucial regulatory role in protein synthesis. Thus, this report might contribute to the basic understanding of mammalian protein translation and sheds some new light on the role of SUMO in this process.
Mesh Terms:
Apoptosis, Cell Proliferation, Cyclin D1, Eukaryotic Initiation Factor-4E, Eukaryotic Initiation Factor-4G, Gene Expression Regulation, Genes, Reporter, HCT116 Cells, Humans, Hydrazones, Multiprotein Complexes, Protein Binding, Protein Biosynthesis, Proto-Oncogene Proteins c-myc, RNA Caps, RNA, Messenger, Small Ubiquitin-Related Modifier Proteins, Thiazoles
PLoS One
Date: Jun. 28, 2014
Download Curated Data For This Publication
242244
Switch View:
  • Interactions 3