GAK: a cyclin G associated kinase contains a tensin/auxilin-like domain.
We have cloned a cDNA encoding a novel association partner of cyclin G by West-Western blotting. The cDNA encodes a protein that harbors a Ser/Thr protein kinase-like catalytic domain at the N-terminal. Hence, we named it GAK (cyclin G-associated kinase). The long C-terminal extension shares homology with tensin and auxilin, ... and contains a leucine zipper region. Co-immunoprecipitation and Western blotting showed that GAK and cyclin G associate together in vivo. GAK also co-precipitated with CDK5, and CDK5 was found to be associated with cyclin G. We also showed by BIAcore analysis that the GAK-cyclin G interaction was direct.
Mesh Terms:
Adaptor Proteins, Vesicular Transport, Amino Acid Sequence, Antibodies, Monoclonal, Blotting, Western, Cloning, Molecular, Cyclin G, Cyclin G1, Cyclin-Dependent Kinases, Cyclins, DNA, Complementary, Kinetics, Microfilament Proteins, Molecular Sequence Data, Nerve Tissue Proteins, Phosphoproteins, Precipitin Tests, Protein-Serine-Threonine Kinases, Recombinant Fusion Proteins
Adaptor Proteins, Vesicular Transport, Amino Acid Sequence, Antibodies, Monoclonal, Blotting, Western, Cloning, Molecular, Cyclin G, Cyclin G1, Cyclin-Dependent Kinases, Cyclins, DNA, Complementary, Kinetics, Microfilament Proteins, Molecular Sequence Data, Nerve Tissue Proteins, Phosphoproteins, Precipitin Tests, Protein-Serine-Threonine Kinases, Recombinant Fusion Proteins
FEBS Lett.
Date: Jan. 27, 1997
PubMed ID: 9013862
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