Effective ACE2 peptide-nanoparticle conjugation and its binding with the SARS-Cov-2 RBD quantified by dynamic light scattering.
The infection of coronavirus initiates with the binding between its spike protein receptor binding domain (RBD) and a human cellular receptor called angiotensin-converting enzyme 2 (ACE2). Here, we construct truncated ACE2 peptide-conjugated gold nanoparticles as antiviral scaffolds and study their binding with the SARS-CoV-2 RBD using dynamic light scattering (DLS). ... Systematic DLS analysis identifies the effective peptide-nanoparticle conjugation and its efficient, specific, and long-lasting multivalent binding towards the RBD with a binding affinity of 41 nM, indicating the potential of this antiviral platform to compete with natural ACE2-RBD interactions for viral blocking and showcasing an accessible approach to measure the binding constants and kinetics.
Mesh Terms:
Angiotensin-Converting Enzyme 2, Dynamic Light Scattering, Molecular Dynamics Simulation, Nanoparticles, Peptide Fragments, Protein Binding, Protein Domains, SARS-CoV-2, Spike Glycoprotein, Coronavirus, Substrate Specificity
Angiotensin-Converting Enzyme 2, Dynamic Light Scattering, Molecular Dynamics Simulation, Nanoparticles, Peptide Fragments, Protein Binding, Protein Domains, SARS-CoV-2, Spike Glycoprotein, Coronavirus, Substrate Specificity
Chem Commun (Camb)
Date: Jul. 15, 2021
PubMed ID: 34190246
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