Systematic analysis of microtubule plus-end networks defines EB-cargo complexes critical for mitosis in budding yeast.

Microtubules are ubiquitous cytoskeletal polymers with essential functions in chromosome segregation, intracellular transport, and cellular morphogenesis. End-binding proteins (EBs) form the nodes of intricate microtubule plus-end interaction networks. Which EB binding partners are most critical for cell division and how cells organize a microtubule cytoskeleton in the absence of an ...
EB protein are open questions. Here, we perform a detailed analysis of deletion and point mutants of the budding yeast EB protein Bim1. We demonstrate that Bim1 executes its key mitotic functions as part of two cargo complexes-Bim1-Kar9 in the cytoplasm and Bim1-Bik1-Cik1-Kar3 in the nucleus. The latter complex acts during initial metaphase spindle assembly and supports tension establishment and sister chromatid biorientation. We demonstrate that engineered plus-end targeting of Cik1-Kar3 and overexpression of the microtubule crosslinker Ase1 restore distinct aspects of the bim1? spindle phenotype. In addition to defining key Bim1-cargo complexes our study also characterizes redundant mechanisms that allow cells to proliferate in the absence of Bim1.
Mesh Terms:
Cell Cycle Proteins, Chromosome Segregation, Microtubule Proteins, Microtubule-Associated Proteins, Microtubules, Mitosis, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Saccharomycetales
Mol Biol Cell
Date: May. 01, 2023
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