Cyclin E associates with BAF155 and BRG1, components of the mammalian SWI-SNF complex, and alters the ability of BRG1 to induce growth arrest.

SWI-SNF complexes have been implicated in transcriptional regulation by chromatin remodeling. We have identified an interaction between two components of the mammalian SWI-SNF complex and cyclin E, an essential cell cycle regulatory protein required for G1/S transition. BRG1 and BAF155, mammalian homologs of yeast SWI2 and SWI3, respectively, are found ...
in cyclin E complexes and are phosphorylated by cyclin E-associated kinase activity. In this report, we show that overexpression of BRG1 causes growth arrest and induction of senescence-associated beta-galactosidase activity, which can be overcome by cyclin E. Our results suggest that cyclin E may modulate the activity of the SWI-SNF apparatus to maintain the chromatin in a transcriptionally permissive state.
Mesh Terms:
Amino Acid Sequence, Cell Cycle, Cell Line, Cell Size, Cyclin E, DNA Helicases, DNA-Binding Proteins, Fungal Proteins, Humans, Molecular Sequence Data, Nuclear Proteins, Phenotype, Retinoblastoma Protein, Saccharomyces cerevisiae Proteins, Trans-Activators, Transcription Factors, Transfection
Mol. Cell. Biol.
Date: Feb. 01, 1999
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