The wis1 protein kinase is a dosage-dependent regulator of mitosis in Schizosaccharomyces pombe.
The wis1+ gene encodes a newly identified mitotic control element in Schizosaccharomyces pombe. It was isolated by virtue of its interaction with the mitotic control genes cdc25, wee1 and win1. The wis1+ gene potentially encodes a 66 kDa protein with homology to the serine/threonine family of protein kinases. wis1+ plays ... an important role in the regulation of entry into mitosis, as it shares with cdc25+ and nim1+/cdr1+ the property of inducing mitosis in a dosage-dependent manner. Increased levels of wis1+ expression cause mitotic initiation to occur at a reduced cell size. Loss of wis1+ function does not prevent vegetative growth and division, though wis1- cells show an elongated morphology, indicating that their entry into mitosis and cell division is delayed relative to wild type cells. wis1- cells undergo a rapid reduction of viability upon entry into stationary phase, suggesting a role for wis1+ in the integration of nutritional sensing with the control over entry into mitosis.
Mesh Terms:
Amino Acid Sequence, Base Sequence, Blotting, Southern, Cloning, Molecular, DNA, Fungal, Fungal Proteins, Mitogen-Activated Protein Kinase Kinases, Mitosis, Molecular Sequence Data, Protein Kinases, RNA, Fungal, Restriction Mapping, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Sequence Homology, Nucleic Acid, Suppression, Genetic
Amino Acid Sequence, Base Sequence, Blotting, Southern, Cloning, Molecular, DNA, Fungal, Fungal Proteins, Mitogen-Activated Protein Kinase Kinases, Mitosis, Molecular Sequence Data, Protein Kinases, RNA, Fungal, Restriction Mapping, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Sequence Homology, Nucleic Acid, Suppression, Genetic
EMBO J.
Date: Dec. 01, 1991
PubMed ID: 1756736
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