Characterization of human dopamine responsive protein DRG-1 that binds to p75NTR-associated cell death executor NADE.

Expression of human dopamine responsive gene-1 (DRG-1) is up-regulated in response to treatment of dopamine in the rat astrocytes. However, its functions are not clear up to now. In the presented studies, DRG-1 was identified to be a conserved gene in the vertebrate and expressed abundantly in human testis, brain ...
and skeletal muscle. DRG-1 was shown to interact with human p75NTR-associated cell death executor (NADE) in vivo and in vitro, and the interaction occurred in cytoplasm. The regions required for the interaction were subsequently mapped to the N-terminal of DRG-1 and the C-terminal of NADE. Furthermore, MTT assay showed that stable expression of DRG-1 in 293 cells could promote cell proliferation, and this promotion was suppressed by overexpression of NADE. In flow cytometry cell cycle analysis, overexpression of DRG-1 in 293 or PC12 cells increased the population of cells in the S phase with a concomitant decrease in G0/G1 population. These findings suggest that DRG-1 may contribute to the dopamine-induced cell growth, which is negatively regulated by NADE.
Mesh Terms:
Amino Acid Sequence, Animals, Apoptosis Regulatory Proteins, COS Cells, Cell Cycle, Cell Division, Cell Proliferation, Chlorocebus aethiops, Chromosome Mapping, Down-Regulation, Flow Cytometry, GTP-Binding Proteins, Glutathione, Humans, Hybridization, Genetic, Immunoprecipitation, Molecular Sequence Data, PC12 Cells, Plasmids, Rats, Reverse Transcriptase Polymerase Chain Reaction, Saccharomyces cerevisiae, Subcellular Fractions, Tetrazolium Salts, Thiazoles, Tissue Distribution, Transfection
Brain Res
Date: Jul. 19, 2006
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