Cold sensitivity of the SARS-CoV-2 spike ectodomain.
The SARS-CoV-2 spike (S) protein, a primary target for COVID-19 vaccine development, presents its receptor binding domain in two conformations, the receptor-accessible 'up' or receptor-inaccessible 'down' states. Here we report that the commonly used stabilized S ectodomain construct '2P' is sensitive to cold temperatures, and this cold sensitivity is abrogated ... in a 'down' state-stabilized ectodomain. Our findings will impact structural, functional and vaccine studies that use the SARS-CoV-2 S ectodomain.
Mesh Terms:
Antibodies, Viral, COVID-19 Vaccines, Cold Temperature, Cryoelectron Microscopy, Enzyme-Linked Immunosorbent Assay, Humans, Protein Denaturation, Protein Domains, Protein Stability, Spike Glycoprotein, Coronavirus, Surface Plasmon Resonance
Antibodies, Viral, COVID-19 Vaccines, Cold Temperature, Cryoelectron Microscopy, Enzyme-Linked Immunosorbent Assay, Humans, Protein Denaturation, Protein Domains, Protein Stability, Spike Glycoprotein, Coronavirus, Surface Plasmon Resonance
Nat Struct Mol Biol
Date: Feb. 01, 2021
PubMed ID: 33402708
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