Chromosome compaction is triggered by an autonomous DNA-binding module within condensin.

The compaction of chromatin into mitotic chromosomes is essential for faithful transmission of the genome during cell division. In eukaryotes, chromosome morphogenesis is regulated by the condensin complex, though the exact mechanism used to target condensin to chromatin and initiate condensation is not understood. Here, we reveal that condensin contains ...
an intrinsically disordered region (IDR) that modulates its association with chromatin in early mitosis and exhibits phase separation. We describe DNA-binding motifs within the IDR that, upon deletion, inflict striking defects in chromosome condensation and segregation, ill-timed condensin turnover on chromatin, and cell death. Importantly, we demonstrate that the condensin IDR can impart cell cycle regulatory functions when transferred to other subunits within the complex, indicating its autonomous nature. Collectively, our study unveils the molecular basis for the initiation of chromosome condensation in early mitosis and how this process ultimately promotes genomic stability and faultless cell division.
Mesh Terms:
Adenosine Triphosphatases, Chromatin, Chromosome Segregation, Chromosomes, DNA, DNA-Binding Proteins, Mitosis, Multiprotein Complexes, Protein Binding, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Cell Rep
Date: Jul. 23, 2024
Download Curated Data For This Publication
252046
Switch View:
  • Interactions 7