The cullin Rtt101 promotes ubiquitin-dependent DNA-protein crosslink repair across the cell cycle.

DNA-protein crosslinks (DPCs) challenge faithful DNA replication and smooth passage of genomic information. Our study unveils the cullin E3 ubiquitin ligase Rtt101 as a DPC repair factor. Genetic analyses demonstrate that Rtt101 is essential for resistance to a wide range of DPC types including topoisomerase 1 crosslinks, in the same ...
pathway as the ubiquitin-dependent aspartic protease Ddi1. Using an in vivo inducible Top1-mimicking DPC system, we reveal the significant impact of Rtt101 ubiquitination on DPC removal across different cell cycle phases. High-throughput methods coupled with next-generation sequencing specifically highlight the association of Rtt101 with replisomes as well as colocalization with DPCs. Our findings establish Rtt101 as a main contributor to DPC repair throughout the yeast cell cycle.
Mesh Terms:
Cell Cycle, Cullin Proteins, DNA Adducts, DNA Repair, DNA Replication, DNA Topoisomerases, Type I, Multienzyme Complexes, Protein Transport, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Ubiquitination
Nucleic Acids Res
Date: Sep. 09, 2024
Download Curated Data For This Publication
252924
Switch View:
  • Interactions 6