The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

The formation of new ribosomes is tightly coordinated with cell growth and proliferation. In eukaryotes, the correct assembly of all ribosomal proteins and RNAs follows an intricate scheme of maturation and rearrangement steps across three cellular compartments: the nucleolus, nucleoplasm, and cytoplasm. We demonstrate that usnic acid, a lichen secondary ...
metabolite, inhibits the maturation of the large ribosomal subunit in yeast. We combine biochemical characterization of pre-ribosomal particles with a quantitative single-particle cryo-EM approach to monitor changes in nucleolar particle populations upon drug treatment. Usnic acid rapidly blocks the transition from nucleolar state B to C of Nsa1-associated pre-ribosomes, depleting key maturation factors such as Dbp10 and hindering pre-rRNA processing. This primary nucleolar block rapidly rebounds on earlier stages of the pathway which highlights the regulatory linkages between different steps. In summary, we provide an in-depth characterization of the effect of usnic acid on ribosome biogenesis, which may have implications for its reported anti-cancer activities.
Mesh Terms:
Benzofurans, Cell Nucleolus, Cryoelectron Microscopy, Lichens, RNA Precursors, RNA, Ribosomal, Ribosomal Proteins, Ribosome Subunits, Large, Ribosome Subunits, Large, Eukaryotic, Ribosomes, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Nat Commun
Date: Aug. 29, 2024
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