Proteome-wide profiling of protein assemblies by cross-linking mass spectrometry.

We describe an integrated workflow that robustly identifies cross-links from endogenous protein complexes in human cellular lysates. Our approach is based on the application of mass spectrometry (MS)-cleavable cross-linkers, sequential collision-induced dissociation (CID)-tandem MS (MS/MS) and electron-transfer dissociation (ETD)-MS/MS acquisitions, and a dedicated search engine, XlinkX, which allows rapid cross-link ...
identification against a complete human proteome database. This approach allowed us to detect 2,179 unique cross-links (1,665 intraprotein cross-links at a 5% false discovery rate (FDR) and 514 interprotein cross-links at 1% FDR) in HeLa cell lysates. We validated the confidence of our cross-linking results by using a target-decoy strategy and mapping the observed cross-link distances onto existing high-resolution structures. Our data provided new structural information about many protein assemblies and captured dynamic interactions of the ribosome in contact with different elongation factors.
Mesh Terms:
Cross-Linking Reagents, Databases, Protein, HeLa Cells, Humans, Models, Molecular, Multiprotein Complexes, Proteome, Proteomics, Reproducibility of Results, Ribosomal Proteins, Tandem Mass Spectrometry
Nat Methods
Date: Dec. 01, 2015
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