Structures of two intermediate filament-binding fragments of desmoplakin reveal a unique repeat motif structure.
Desmosomes are intercellular junctions in which cadherin cell adhesion molecules are linked to the intermediate filament (IF) system. Desmoplakin is a member of the plakin family of IF-binding proteins. The C-terminal domain of desmoplakin (DPCT) mediates binding to IFs in desmosomes. The DPCT sequence contains three regions, termed A, B ... and C, consisting of 4.5 copies of a 38-amino acid repeat motif. We demonstrate that these regions form discrete subdomains that bind to IFs and report the crystal structures of domains B and C. In contrast to the elongated structures formed by other kinds of repeat motifs, the plakin repeats form a globular structure with a unique fold. A conserved basic groove found on the domain may represent an IF-binding site.
Mesh Terms:
Amino Acid Sequence, Binding Sites, Crystallography, X-Ray, Cytoskeletal Proteins, Desmoplakins, Humans, Intermediate Filaments, Models, Molecular, Molecular Sequence Data, Peptide Fragments, Peptide Mapping, Protein Structure, Tertiary, Recombinant Proteins, Repetitive Sequences, Nucleic Acid, Sequence Homology, Amino Acid, Static Electricity
Amino Acid Sequence, Binding Sites, Crystallography, X-Ray, Cytoskeletal Proteins, Desmoplakins, Humans, Intermediate Filaments, Models, Molecular, Molecular Sequence Data, Peptide Fragments, Peptide Mapping, Protein Structure, Tertiary, Recombinant Proteins, Repetitive Sequences, Nucleic Acid, Sequence Homology, Amino Acid, Static Electricity
Nat. Struct. Biol.
Date: Aug. 01, 2002
PubMed ID: 12101406
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