Nuclear receptor coactivator thyroid hormone receptor-binding protein (TRBP) interacts with and stimulates its associated DNA-dependent protein kinase.
Nuclear receptors mediate gene activation through ligand-dependent interaction with coactivators. We previously cloned and characterized thyroid hormone receptor-binding protein, TRBP (NcoA6: AIB3/ASC-2/RAP250/PRIP/TRBP/NRC), as an LXXLL-containing coactivator that associates with coactivator complexes through its C terminus. To search for protein factors involved in TRBP action, we identified a distinct set of ... proteins from HeLa nuclear extract that interacts with the C terminus of TRBP. Analysis by mass spectrometric protein sequencing revealed a DNA-dependent protein kinase (DNA-PK) complex including its catalytic subunit and regulatory subunits, Ku70 and Ku86. DNA-PK is a heterotrimeric nuclear phosphatidylinositol 3-kinase that functions in DNA repair, recombination, and transcriptional regulation. DNA-PK phosphorylates TRBP at its C-terminal region, which directly interacts with Ku70 but not Ku86 in vitro. In addition, in the absence of DNA, TRBP itself activates DNA-PK, and the TRBP-stimulated DNA-PK activity has an altered phosphorylation pattern from DNA-stimulated activity. An anti-TRBP antibody inhibits TRBP-induced kinase activity, suggesting that protein content of TRBP is responsible for the stimulation of DNA-independent kinase activity. Furthermore, in DNA-PK-deficient scid cells, TRBP-mediated transactivation is significantly impaired, and nuclear localization of TRBP is altered. The activation of DNA-PK in the absence of DNA ends by the coactivator TRBP suggests a novel mechanism of coactivator-stimulated DNA-PK phosphorylation in transcriptional regulation.
Mesh Terms:
1-Phosphatidylinositol 3-Kinase, Carrier Proteins, DNA-Activated Protein Kinase, DNA-Binding Proteins, Electrophoresis, Polyacrylamide Gel, Enzyme Activation, Fluorescent Antibody Technique, Hela Cells, Humans, Membrane Proteins, Nuclear Proteins, Phosphorylation, Protein Binding, Protein-Serine-Threonine Kinases, Recombination, Genetic, Substrate Specificity, Thyroid Hormones, Transcription, Genetic
1-Phosphatidylinositol 3-Kinase, Carrier Proteins, DNA-Activated Protein Kinase, DNA-Binding Proteins, Electrophoresis, Polyacrylamide Gel, Enzyme Activation, Fluorescent Antibody Technique, Hela Cells, Humans, Membrane Proteins, Nuclear Proteins, Phosphorylation, Protein Binding, Protein-Serine-Threonine Kinases, Recombination, Genetic, Substrate Specificity, Thyroid Hormones, Transcription, Genetic
J. Biol. Chem.
Date: Mar. 28, 2003
PubMed ID: 12519782
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