A DNA-topoisomerase-II-binding protein with eight repeating regions similar to DNA-repair enzymes and to a cell-cycle regulator.
A two-hybrid system was used to isolate factors that interact with the C-terminal region of DNA topoisomerase IIbeta. A positive clone isolated from a HeLa cDNA library encoded 1522 amino acid residues (molecular mass 170670). The protein, designated topoisomerase-IIbeta-binding protein 1 (TopBP1), interacted with the C-terminal region of topoisomerase IIbeta ... synthesized in vitro. A database search indicated that TopBP1 possessed eight regions similar to regions of Rad4, Cut5, Ect2, Rev1 and X-ray repair cross-complementing 1 (XRCC1) proteins and a region similar to auto-modification sites of poly(ADP-ribose) polymerase, suggesting that TopBP1 supported catalytic reactions of topoisomerase II through transient breakages of DNA strands.
Mesh Terms:
Amino Acid Sequence, Base Sequence, Blotting, Northern, Carrier Proteins, Cloning, Molecular, DNA Ligases, DNA Topoisomerases, Type II, DNA-Binding Proteins, Escherichia coli, Hela Cells, Humans, Molecular Sequence Data, Nuclear Proteins, Protein Binding, RNA, Messenger, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Sequence Analysis, DNA, Sequence Homology, Amino Acid
Amino Acid Sequence, Base Sequence, Blotting, Northern, Carrier Proteins, Cloning, Molecular, DNA Ligases, DNA Topoisomerases, Type II, DNA-Binding Proteins, Escherichia coli, Hela Cells, Humans, Molecular Sequence Data, Nuclear Proteins, Protein Binding, RNA, Messenger, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Sequence Analysis, DNA, Sequence Homology, Amino Acid
Eur. J. Biochem.
Date: Dec. 15, 1997
PubMed ID: 9461304
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