The fission yeast dis2+ gene required for chromosome disjoining encodes one of two putative type 1 protein phosphatases.

S. pombe dis mutants block mitotic chromosome disjunction in a manner reminiscent of aneuploidy formation, and belong to three distinct genes, dis1-dis3. We cloned two independent genomic DNAs that complemented both the cold-sensitive and caffeine-hypersensitive phenotype of dis2-11. These genes, dis2+ and a suppressor sds21+, encode proteins (calculated MW 37,000) ...
with similar predicted amino acid sequences. dis2+ and sds21+ have overlapping functions, and disruptants are lethal only when both genes are disrupted. The gene products identified by anti-dis2 serum are enriched in nuclei. By hybridization, we obtained two cDNA clones from mouse and one genomic clone from S. cerevisiae; the latter complements S. pombe dis2-11. These dis2+ and similar polypeptides of yeasts and mouse are found to be highly homologous (75%-90% identical) to rabbit protein phosphatase 1. The implications of these findings are discussed with regard to mitotic control.
Mesh Terms:
Alleles, Amino Acid Sequence, Animals, Base Sequence, Caffeine, Chromosome Mapping, Cloning, Molecular, Cold Temperature, DNA, Fungal, Fluorescent Antibody Technique, Fungal Proteins, Genes, Fungal, Genes, Lethal, Genetic Complementation Test, Mice, Molecular Sequence Data, Molecular Weight, Nondisjunction, Genetic, Phosphoprotein Phosphatases, Protein Phosphatase 1, Saccharomyces cerevisiae, Saccharomycetales, Schizosaccharomyces
Cell
Date: Jun. 16, 1989
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