Localisation and regulation of the eIF4E-binding protein 4E-BP3.
The cap-binding protein eIF4E-binding protein 3 (4E-BP3) was identified some years ago, but its properties have not been investigated in detail. In this report, we investigated the regulation and localisation of 4E-BP3. We show that 4E-BP3 is present in the nucleus as well as in the cytoplasm in primary T ... cells, HEK293 cells and HeLa cells. 4E-BP3 was associated with eIF4E in both cell compartments. Furthermore, 4E-BP3/eIF4E association in the cytoplasm was regulated by serum or interleukin-2 starvation in the different cell types. Rapamycin did not affect the association of eIF4E with 4E-BP3 in the cytoplasm or in the nucleus.
Mesh Terms:
Blotting, Western, Carrier Proteins, Cell Line, Cell Nucleus, Culture Media, Serum-Free, Cytoplasm, Electrophoresis, Polyacrylamide Gel, Eukaryotic Initiation Factor-4E, Gene Expression Regulation, Hela Cells, Humans, Immunosuppressive Agents, Interleukin-2, Peptides, Protein Binding, RNA Cap Analogs, Sirolimus, T-Lymphocytes, Transfection
Blotting, Western, Carrier Proteins, Cell Line, Cell Nucleus, Culture Media, Serum-Free, Cytoplasm, Electrophoresis, Polyacrylamide Gel, Eukaryotic Initiation Factor-4E, Gene Expression Regulation, Hela Cells, Humans, Immunosuppressive Agents, Interleukin-2, Peptides, Protein Binding, RNA Cap Analogs, Sirolimus, T-Lymphocytes, Transfection
FEBS Lett.
Date: Dec. 18, 2002
PubMed ID: 12482586
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