Protection against Fas/APO-1- and tumor necrosis factor-mediated cell death by a novel protein, sentrin.
Fas/APO-1 and TNF receptor 1 share a common signaling motif in their cytoplasmic tail called the "death domain." Using the death domain as bait in the yeast two-hybrid system, several death domain-containing proteins that participate in cell death signaling have been identified. Here we report the isolation of a novel ... protein, sentrin, which interacts with Fas/APO-1 and TNF receptor 1 but not with FADD/MORT1 or CD40. Two-hybrid interaction assays reveal that sentrin associates only with the signal-competent forms of Fas/APO-1 or TNF receptor 1 death domains. Sentrin is a novel protein of 101 amino acids with homology to ubiquitin, Nedd8, and a Saccharomyces cerevisiae protein, Smt3. When overexpressed, sentrin provides protection against both anti-Fas/APO-1 and TNF-induced cell death.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Amino Acid Sequence, Animals, Antigens, CD95, Apoptosis, Base Sequence, Carrier Proteins, Fas-Associated Death Domain Protein, Fungal Proteins, L Cells (Cell Line), Mice, Molecular Sequence Data, Protein Binding, Protein Conformation, Receptors, Tumor Necrosis Factor, Recombinant Proteins, Repressor Proteins, SUMO-1 Protein, Saccharomyces cerevisiae Proteins, Small Ubiquitin-Related Modifier Proteins, Tumor Necrosis Factor-alpha, Ubiquitins
Adaptor Proteins, Signal Transducing, Amino Acid Sequence, Animals, Antigens, CD95, Apoptosis, Base Sequence, Carrier Proteins, Fas-Associated Death Domain Protein, Fungal Proteins, L Cells (Cell Line), Mice, Molecular Sequence Data, Protein Binding, Protein Conformation, Receptors, Tumor Necrosis Factor, Recombinant Proteins, Repressor Proteins, SUMO-1 Protein, Saccharomyces cerevisiae Proteins, Small Ubiquitin-Related Modifier Proteins, Tumor Necrosis Factor-alpha, Ubiquitins
J. Immunol.
Date: Nov. 15, 1996
PubMed ID: 8906799
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