Fibroblast growth factor-2 interacts with free ribosomal protein S19.
Exogenous FGF-2 added to cells is internalized and part of it translocates to the nucleus of the cells. To get a better understanding of the FGF-2-induced signaling pathway, we looked for proteins associated with FGF-2 in the cytoplasm of the target cells. We first used the GST-FGF-2 to isolate cytoplasmic ... proteins complexes containing FGF-2 from S100 extract (supernatant 100,000g). Among the retrieved proteins, we focused our studies on RPS19, a protein of the 40S small ribosomal subunit. We showed that FGF-2 interacts directly with RPS19 in vitro. Second, we coimmunoprecipitated RPS19 and FGF-2 from a S240 extract (240,000g supernatant) prepared from FGF-2-stimulated cells and devoid of 40S ribosomal subunit. The result of these experiments suggest that a pool of free RPS19 exists in cells and that FGF-2 interacts in vivo with free RPS19.
Mesh Terms:
3T3 Cells, Amino Acid Sequence, Animals, Biotinylation, Blotting, Northern, Chromatography, Affinity, Cytoplasm, Dose-Response Relationship, Drug, Fibroblast Growth Factor 2, Gene Deletion, Glutathione Transferase, Humans, Mice, Molecular Sequence Data, Plasmids, Precipitin Tests, Protein Binding, Recombinant Proteins, Ribosomal Proteins, Ribosomes, Signal Transduction
3T3 Cells, Amino Acid Sequence, Animals, Biotinylation, Blotting, Northern, Chromatography, Affinity, Cytoplasm, Dose-Response Relationship, Drug, Fibroblast Growth Factor 2, Gene Deletion, Glutathione Transferase, Humans, Mice, Molecular Sequence Data, Plasmids, Precipitin Tests, Protein Binding, Recombinant Proteins, Ribosomal Proteins, Ribosomes, Signal Transduction
Biochem. Biophys. Res. Commun.
Date: Nov. 30, 2001
PubMed ID: 11716516
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