PACS-1 defines a novel gene family of cytosolic sorting proteins required for trans-Golgi network localization.
We report the role of one member of a novel gene family, PACS-1, in the localization of trans-Golgi network (TGN) membrane proteins. PACS-1 directs the TGN localization of furin by binding to the protease's phosphorylated cytosolic domain. Antisense studies show TGN localization of furin and mannose-6-phosphate receptor, but not TGN46, ... is strictly dependent on PACS-1. Analyses in vitro and in vivo show PACS-1 has properties of a coat protein and connects furin to components of the clathrin-sorting machinery. Cell-free assays indicate TGN localization of furin is directed by a PACS-1-mediated retrieval step. Together, these findings explain a mechanism by which membrane proteins in mammalian cells are localized to the TGN.
Mesh Terms:
Adaptor Protein Complex alpha Subunits, Adaptor Proteins, Vesicular Transport, Amino Acid Sequence, Animals, Capsid, Carrier Proteins, Cell Line, Clathrin, Cloning, Molecular, Cytosol, Furin, Golgi Apparatus, Membrane Proteins, Mice, Molecular Sequence Data, Multigene Family, Phosphorylation, Receptor, IGF Type 2, Subtilisins, Vesicular Transport Proteins
Adaptor Protein Complex alpha Subunits, Adaptor Proteins, Vesicular Transport, Amino Acid Sequence, Animals, Capsid, Carrier Proteins, Cell Line, Clathrin, Cloning, Molecular, Cytosol, Furin, Golgi Apparatus, Membrane Proteins, Mice, Molecular Sequence Data, Multigene Family, Phosphorylation, Receptor, IGF Type 2, Subtilisins, Vesicular Transport Proteins
Cell
Date: Jul. 24, 1998
PubMed ID: 9695949
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