Interaction of p59fyn with interferon-activated Jak kinases.
During IFN alpha stimulation, p59(fyn) associates with the Type I IFNR-associated Tyk-2 kinase in several human hematopoietic cell lines in vivo. This interaction is direct, and is mediated by the SH2 domain in p59(fyn), as shown by binding studies using glutathione-S-transferase fusion proteins and far western blots. Furthermore, in response ... to IFN alpha-treatment of cells, the SH2 domain of Fyn interacts with the Tyk-2-associated c-cbl proto-oncogene product. In a similar manner, during IFN gamma stimulation, p59(fyn) associates via its SH2 domain with the activated form of the IFN gamma-dependent Jak-2 kinase. These data suggest that p59(fyn) is a common element in IFN alpha and IFN gamma signaling, and is selectively engaged by the Type I or II IFN receptors via specific interactions with distinct Jak kinases.
Mesh Terms:
Antibodies, Monoclonal, Blotting, Western, Glutathione Transferase, Humans, Interferon Type I, Recombinant, Interferon-gamma, Recombinant, Janus Kinase 1, Janus Kinase 2, Phosphotyrosine, Protein-Tyrosine Kinases, Proteins, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-cbl, Proto-Oncogene Proteins c-fyn, Recombinant Fusion Proteins, Signal Transduction, TYK2 Kinase, Tumor Cells, Cultured, Ubiquitin-Protein Ligases, src Homology Domains
Antibodies, Monoclonal, Blotting, Western, Glutathione Transferase, Humans, Interferon Type I, Recombinant, Interferon-gamma, Recombinant, Janus Kinase 1, Janus Kinase 2, Phosphotyrosine, Protein-Tyrosine Kinases, Proteins, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-cbl, Proto-Oncogene Proteins c-fyn, Recombinant Fusion Proteins, Signal Transduction, TYK2 Kinase, Tumor Cells, Cultured, Ubiquitin-Protein Ligases, src Homology Domains
Biochem. Biophys. Res. Commun.
Date: Jun. 09, 1997
PubMed ID: 9196040
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