The intranuclear localization and function of YT521-B is regulated by tyrosine phosphorylation.
YT521-B is a ubiquitously expressed nuclear protein that changes alternative splice site usage in a concentration dependent manner. YT521-B is located in a dynamic nuclear compartment, the YT body. We show that YT521-B is tyrosine phosphorylated by c-Abl in the nucleus. The protein shuttles between nucleus and cytosol, where it ... can be phosphorylated by c-Src or p59(fyn). Tyrosine phosphorylation causes dispersion of YT521-B from YT bodies to the nucleoplasm. Whereas YT bodies are soluble in non-denaturing buffers, the phosphorylated, dispersed form is non-soluble. Non-phosphorylated YT521-B changes alternative splice site selection of the IL-4 receptor, CD44 and SRp20, but phosphorylation of c-Abl minimizes this concentration dependent effect. We propose that tyrosine phosphorylation causes sequestration of YT521-B in an insoluble nuclear form, which abolishes the ability of YT521-B to change alternative splice sites.
Mesh Terms:
Alternative Splicing, Cell Nucleus, Cytoplasm, Genes, Reporter, Humans, Microscopy, Fluorescence, Nerve Tissue Proteins, Phosphorylation, Protein-Tyrosine Kinases, Proto-Oncogene Proteins c-abl, RNA Precursors, RNA-Binding Proteins, Receptors, Interleukin-4, Transfection, Tyrosine
Alternative Splicing, Cell Nucleus, Cytoplasm, Genes, Reporter, Humans, Microscopy, Fluorescence, Nerve Tissue Proteins, Phosphorylation, Protein-Tyrosine Kinases, Proto-Oncogene Proteins c-abl, RNA Precursors, RNA-Binding Proteins, Receptors, Interleukin-4, Transfection, Tyrosine
Hum. Mol. Genet.
Date: Aug. 01, 2004
PubMed ID: 15175272
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