Genes involved in sister chromatid separation are needed for B-type cyclin proteolysis in budding yeast.

B-type cyclin destruction is necessary for exit from mitosis and the initiation of a new cell cycle. Through the isolation of mutants, we have identified three essential yeast genes, CDC16, CDC23, and CSE1, which are required for proteolysis of the B-type cyclin CLB2 but not of other unstable proteins. cdc23-1 ...
mutants are defective in both entering and exiting anaphase. Their failure to exit anaphase can be explained by defective cyclin proteolysis. CDC23 is required at the metaphase/anaphase transition to separate sister chromatids, and we speculate that it might promote proteolysis of proteins that hold sister chromatids together. Proteolysis of CLB2 is initiated in early anaphase, but a fraction of CLB2 remains stable until anaphase is complete.
Mesh Terms:
Anaphase, Blotting, Western, Cell Cycle Proteins, Chromatids, Cyclins, Fungal Proteins, Gene Deletion, Genes, Fungal, Ligases, Mitosis, Models, Biological, Mutagenesis, Nuclear Proteins, Nucleocytoplasmic Transport Proteins, Precipitin Tests, Saccharomyces cerevisiae Proteins, Selection, Genetic, Ubiquitin-Conjugating Enzymes, Ubiquitin-Protein Ligase Complexes, Yeasts
Date: Apr. 21, 1995
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