Genes involved in sister chromatid separation are needed for B-type cyclin proteolysis in budding yeast.
B-type cyclin destruction is necessary for exit from mitosis and the initiation of a new cell cycle. Through the isolation of mutants, we have identified three essential yeast genes, CDC16, CDC23, and CSE1, which are required for proteolysis of the B-type cyclin CLB2 but not of other unstable proteins. cdc23-1 ... mutants are defective in both entering and exiting anaphase. Their failure to exit anaphase can be explained by defective cyclin proteolysis. CDC23 is required at the metaphase/anaphase transition to separate sister chromatids, and we speculate that it might promote proteolysis of proteins that hold sister chromatids together. Proteolysis of CLB2 is initiated in early anaphase, but a fraction of CLB2 remains stable until anaphase is complete.
Mesh Terms:
Anaphase, Blotting, Western, Cell Cycle Proteins, Chromatids, Cyclins, Fungal Proteins, Gene Deletion, Genes, Fungal, Ligases, Mitosis, Models, Biological, Mutagenesis, Nuclear Proteins, Nucleocytoplasmic Transport Proteins, Precipitin Tests, Saccharomyces cerevisiae Proteins, Selection, Genetic, Ubiquitin-Conjugating Enzymes, Ubiquitin-Protein Ligase Complexes, Yeasts
Anaphase, Blotting, Western, Cell Cycle Proteins, Chromatids, Cyclins, Fungal Proteins, Gene Deletion, Genes, Fungal, Ligases, Mitosis, Models, Biological, Mutagenesis, Nuclear Proteins, Nucleocytoplasmic Transport Proteins, Precipitin Tests, Saccharomyces cerevisiae Proteins, Selection, Genetic, Ubiquitin-Conjugating Enzymes, Ubiquitin-Protein Ligase Complexes, Yeasts
Cell
Date: Apr. 21, 1995
PubMed ID: 7736579
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