NMDA receptor function is regulated by the inhibitory scaffolding protein, RACK1.

Ernest Gallo Clinic and Research Center, University of California, San Francisco, CA 94110-3518, USA.
Phosphorylation regulates the function of ligand-gated ion channels such as the N-methyl d-aspartate (NMDA) receptor. Here we report a mechanism for modulation of the phosphorylation state and function of the NMDA receptor via an inhibitory scaffolding protein, RACK1. We found that RACK1 binds both the NR2B subunit of the NMDA receptor and the nonreceptor protein tyrosine kinase, Fyn. RACK1 inhibits Fyn phosphorylation of NR2B and decreases NMDA receptor-mediated currents in CA1 hippocampal slices. Peptides that disrupt the interactions between RACK1, NR2B, and Fyn induce phosphorylation and potentiate NMDA receptor-mediated currents. Therefore, RACK1 is a regulator of NMDA receptor function and may play a role in synaptic plasticity, addiction, learning, and memory.
Mesh Terms:
Amino Acid Sequence, Animals, DNA, Complementary, Dose-Response Relationship, Drug, Electrophysiology, Escherichia coli, GTP-Binding Proteins, Gene Expression Regulation, Hippocampus, Humans, Male, Molecular Sequence Data, Neoplasm Proteins, Peptides, Phosphorylation, Precipitin Tests, Protein Binding, Protein Biosynthesis, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-fyn, Rats, Rats, Sprague-Dawley, Receptors, Cell Surface, Receptors, N-Methyl-D-Aspartate, Recombinant Proteins, Two-Hybrid System Techniques
Proc. Natl. Acad. Sci. U.S.A. Apr. 16, 2002; 99(8);5710-5 [PUBMED:11943848]
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