Autophosphorylation activity and association with Src family kinase of Sky receptor tyrosine kinase.

Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.
"Sky" is a putative receptor tyrosine kinase predominantly expressed in the brain. Sky, like Axl/Ufo/Ark and c-Eyk, has an extracellular domain composed of two immunoglobulin-like domains and two fibronectin type III domains. Immunoblot analysis using an antibody raised against a C-terminal peptide of Sky identified a 98-kDa Sky protein in COS cells transfected with sky cDNA (COS/sky cells). A 98-kDa protein in the immunoprecipitates with anti-Sky antibody was autophosphorylated on tyrosine, by in vitro kinase reaction. When the lysates of COS/sky cells were immunoprecipitated with anti-Sky antibody and immunoblotted with an anti-phosphotyrosine antibody, a 60-kDa phosphotyrosine-containing protein, in addition to the tyrosine-phosphorylated Sky, was detected. Using the anti-Src antibody, which is reactive to Src, Fyn and Yes, we obtained evidence for an association between the Src family tyrosine kinase and the tyrosine-phosphorylated Sky receptor. These results suggest that the Src family kinase may play an important role in signal transduction of the Sky receptor.
Mesh Terms:
Amino Acid Sequence, Animals, Blotting, Western, Cells, Cultured, Cercopithecus aethiops, Humans, Ligands, Molecular Sequence Data, Multigene Family, Peptides, Phosphorylation, Precipitin Tests, Proto-Oncogene Proteins pp60(c-src), Receptor Protein-Tyrosine Kinases, Signal Transduction
Biochem. Biophys. Res. Commun. Apr. 17, 1995; 209(2);656-63 [PUBMED:7537495]
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